This glossary contains a number of definitions from the following publication:
J. M. Cock; Tessmar-Raible, K.; Boyen, C.; Viard, F. (Eds.), Introduction to Marine Genomics, vol. 1, 1st ed., Advances in Marine Genomics Vol. (Springer, 2010). Available from: http://www.springer.com/life+
Term | Definition |
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RAPD |
Random Amplification of Polymorphic DNA; DNA fragments randomly amplified by PCR from genomic DNA with short primers (8-12 nucleotides) of arbitrary nucleotide sequence. |
RAST |
Rapid Annotation using Subsystem Technology. |
Replicate |
A repetition of the same experimental measurement, The replicate can either be technical, where the same biological extract is analyzed twice, eg. by using the same mRNA for multiple microarrays; or it can be a biological replicate, when for example sample material is harvested from different individuals grown under the same conditions. |
Reporter/Probe |
An oligonucleotide attached to the surface of a microarray. |
RNA interference (RNAI) |
A genetic mechanism in which double-stranded RNA is cleaved into small fragments and acts a signal to either initiate the degradation of a complementary messenger RNA or to interfere with its translation. |
ROS |
Reactive Oxygen species; Small molecules or ions formed by the incomplete one-electron reduction of oxygen; ROS contribute to oxidative stress/damage of DNA, lipids (oxidations of polydesaturated fatty acids), proteins (oxidations of amino acids) and inactivate specific enzymes (oxidation of co-factors). |